专利名称：应用于原核生物和真核生物的基于核酸探针的诊断检验的制作方法ssrA基因编码小而稳定的高拷贝数的转录RNA序列(tmRNA)，该基因在所有的细菌中都存在，并且近来证实这种基因还存在于叶绿体和硅藻体中。tmRNA具有tRNA和mRNA分子的双重功能，并且参与补救失去终止密码子的截短的mRNA，促进截短的多肽在蛋白酶进行降解之前完成通读翻译(Keiler，K.C.et al.(1996)，Science，271，990-993)。tmRNA的这种特殊功能使得研究者开始对其分子的二级结构与功能之间的关系进行分析。这些研究集中在来自不同微生物的tmRNA的二级结构的保守性以及这种结构保守性在进化上的意义和功能相关性。Matveeva等(Matveeva.O et al(1998)，Vol.16，No.13，1374-1375)用tmRNA作为含有二级结构的RNA的模型研究了寡核苷酸与RNA分子的结合。研究没有实现鉴定tmRNA上的位点以及设计用于治疗目的的反义寡核苷酸的目标。现在用于细菌诊断的核酸靶点/探针的数量非常有限。因此，当前需要优先考虑鉴定出用于诊断目的的DNA和RNA靶点。用于发展探针的靶核苷酸序列例如可以是质粒，核糖体RNA基因，基因之间的序列，编码毒力因子的基因或随机基因组DNA片段。此外许多RNA分子已被人描述为可以作为基于RNA的检测的靶点如核糖体RNA和RNA酶P。任何基于核酸探针的检验都需要对当前所研究的真核生物和原核生物的核酸序列的特征有清楚的了解。对原核生物或真核生物进行可靠的检测，所用核酸探针应该为高度特异性(即，和其它生物的核酸序列没有交叉反应)和高度敏感性(即，该生物的所有或多数株系都应该与探针反应并被检测到)。所以，优选的靶点序列应存在于目标生物体的所有的株系之中。该序列应具有显著的序列变异性以保证将目标物种与其它亲缘相近的物种能够区分开；但另一方面，应具有足够的序列保守性以保证能够检测出目标物种的所有株系。一般来说，对一个核酸序列的精确鉴定是进行核酸探针检验的基础，但精确鉴定是费时、困难和不确定的。到目前为止只有为数不多的几种方法能够用于发展核酸探针来进行较广范围的微生物检测。已经被证实可以作为开发探针中潜在可用的靶点的核酸序列包括如rRNA基因和RNA以及rRNA 16S/23S基因间序列。多数核酸探针/靶点检验都集中于用细菌的高拷贝核糖体RNA(rRNA)和rRNA 16S/23S间隔区序列(欧洲专利No.0 395 292)来进行检测和鉴定。市场上已经有的很多成功的商品化细菌检测试剂盒是基于rRNA探针/靶点来检测多种微生物。这包括由Genprobe Inc.(SanDiego California)出售的基于16S rRNA的一系列商品化探针诊断试剂盒，以及由Innogenetics N.V.(Ghent，Belgium)出售的基于16S/23S间隔区序列的DNA探针。但是大多数这种诊断试剂盒都有其局限性，包括由于靶点序列的低拷贝数造成的敏感性缺乏以及很多情况下由亲缘相近的生物体之间的序列一致性引起的特异性缺乏。能够直接应用于样品中指示其中存在的任何活的微生物的基于核酸的检测方法在食品、工业、环境和医学中的应用都有重大意义。基于DNA的检测方法的一个缺点是他们不能区分活的和死的生物体。一些研究致力于用rRNA和mRNA作为细胞存活的指示剂(Sheridan，G.E.C.et al.(1998)Applied and Environmental Microbiology，64，1313-1318)。但是这些序列并不是合适的靶点，因为rRNA和mRNA在细菌死后在其细胞中还会存在48小时。随着基于核酸的类似微阵列形式的能同时对多核酸靶点进行监测的技术的出现，现在需要鉴别和表征新核酸序列作为探针和/或靶点区域来检测和鉴别活的真核细胞和原核细胞。发明公开本发明提供ssrA基因或其片段作为对原核生物或真核生物的核酸探针检验中的靶点区域。
因此，本发明适用于除病毒外的所有生物体。
尚没有有关使用ssrA基因的区域作为靶点区来检测和鉴别原核生物和真核生物的其它核酸探针检验及其相应的优势的报道。
根据本发明的一个实施方案，ssrA基因分子中与从DNA分子5’末端起的高同源性区相对应的片段能够作为通用靶点区域。
在本发明的另一个实施方案中，ssrA基因分子中与从DNA分子3’末端起的高同源性区相对应的片段能够作为通用靶点区域。
在本发明的一个进一步的实施方案中，ssrA基因分子中与低同源性区域相对应的片段能够作为区分不同物种的核酸探针检验的靶点区。
在本发明的另一个进一步的实施方案中，ssrA基因分子中与低同源性区域相对应的片段能够作为用来产生物属特异性探针的靶点区。
如下文所描述，不同生物体的ssrA基因的核酸序列的对比表明这些分子的5’和3’区具有高度同源性，所以可以用为通用靶点序列区。在亲缘相近的生物体间，ssrA基因比其它细菌高拷贝数RNA有更显著程度的序列变异性。这些变异区可作为区分不同物种的核酸检验的理想靶点。
本发明还提供了tmRNA即ssrA基因的RNA转录序列或其片段用作为对原核生物或真核生物的核酸探针检验中的靶点区域。
根据本发明这一方面的一个实施方案，tmRNA分子中与从tmRNA5’末端起的一段高同源性区相对应的片段能够作为通用靶点区。
可替代地，tmRNA中与从tmRNA 3’末端起的一段高同源性区相对应的片段能够作为通用靶点区。
根据本发明这一方面的一个进一步实施方案，tmRNA分子中与低同源性区相对应的片段能够作为区分不同物种的核酸探针检验的靶点区。
根据本发明的另一个进一步的实施方案，tmRNA分子中与低同源性区域相对应的片段能够作为用来产生属特异性探针的靶点区。
根据本发明的核酸探针(DNA或RNA)典型地含有来自于ssrA基因或tmRNA转录序列或它们的互补序列的至少10个核苷酸，这些探针被用于真核生物或原核生物的核酸探针杂交检验。探针与它的互补序列的杂交典型地通过在核酸探针上标记上放射性或非放射性(如比色的或荧光的)标记物来揭示。
在优选的实施方案中，所述的ssrA基因片段或tmRNA片段可作为用于扩增过程中所用的引物的基础。
与ssrA基因或tmRNA序列的5’或3’区相对应的通用寡核苷酸引物可按照本发明使用，以便从广泛的细菌谱中扩增ssrA基因或它所编码的tmRNA，有助于实现大范围生物体的同时扩增，同时能使特异性的核酸探针发生杂交并进行检测。
优选地，扩增过程的产物可以作为核酸探针检验中的靶点区。
更加优选地，tmRNA分子的cDNA转录序列被作为探针用于核酸杂交检验。
这种检测可以在体外完成或在原位完成。
这里所定义的靶点区能够作为区分活的和死的原核生物或真核生物的检测的基础。
与细菌死后存在于其细胞中的rRNA和mRNA不同，tmRNA在死亡的生物体中迅速降解。因此，tmRNA能够作为有用的靶点来区分死的和活的原核生物或真核生物，即，通过核酸探针直接与分离的细菌RNA杂交或者通过将RNA扩增并将扩增产物和核酸探针杂交来将死的和活的原核生物或真核生物进行区分。
优选地，靶点区以多种探针形式使用，以大范围地检测和/或鉴定原核生物或真核生物。
根据本发明的ssrA基因探针或tmRNA转录序列探针可以被连接到微阵列基因芯片系统上，以大范围地对原核生物或真核生物进行高通量的检测和鉴定。
根据本发明的靶点区能够作为试验中的探针来检测目标样品中的原核生物或真核生物。
上述目标样品包括生物样品例如呼吸道的组织样品，泌尿生殖道的组织样品或胃肠道的组织样品，或者体液如血液和血液组分，唾液或脑脊液。
根据本发明的检验还可以在其它样品中完成，如食品样品，环境样品包括空气、水、海洋和土壤样品，以及植物和动物来源的样品。
根据本发明，ssrA基因或tmRNA转录序列的片段可被用于获得原核生物或真核生物的DNA图谱的检验中，并因此能够区分同一物种的不同株系。
核酸序列的对比表明在每一物种之内都会在ssrA基因和tmRNA转录序列内发生序列变异。种内序列变异性能被用来区分同一物种的不同株系来研究流行病学、追踪例如爆发时的感染媒介，或者进行种群研究。
本发明的其它应用包括用ssrA基因，tmRNA转录序列或与其互补的DNA序列或其片段来设计用于治疗目的的试剂来对抗感染性的原核生物或真核生物。
这些试剂可包括反义mRNA或寡核苷酸、核酶和拮抗性多肽并且可以任何药学上适用的形式来使用。
因此，本发明通过使用tmRNA靶点能被用于只检测生物样品中存活的生物体。因此，在任何抗感染药物治疗期间和之后，tmRNA靶点都能够用于监测对于这些特异性感染媒介(如抗微生物和/或抗寄生虫治疗)的治疗效率。
在一个实施方案中，靶点区被用来监测药物对感染媒介的治疗效率。
在另一个实施方案中，靶点区被用来监测胃肠道中促进健康的微生物的存活率和水平。
本发明的这一方面涉及，例如，将包含于如酸乳酪或其它促进健康的食品中的促进健康(益生的)的微生物菌群引入肠道。能够持续监测这些微生物的存在及水平对于保证它们持续促进健康的功能是有必要的。tmRNA区能够作为检测存活的微生物，例如在粪便中的微生物的靶点，以监测促进健康的生物体的存在。
在一个进一步的实施方案中，检验被用于对原核生物或真核生物进行定量分析。
当使用探针杂交和/或体外扩增来检测样品中的微生物时，可以通过信号强度来测定所存在的微生物的数量。实时体外扩增的方法能被用来对样品中的生物体进行定量分析。因此，对样品中的生物体进行定量分析的能力对用于治疗目的的临床情况是非常重要的，如监测抗生素治疗或其它治疗手段或治疗效率。
本发明的另一个进一步的应用是使用ssrA基因序列的数据库来鉴定原核生物或真核生物。
本发明提供一系列用于ssrA基因和tmRNA序列5’和3’端的同源区和变异区的探针，这些探针来自于这些序列或这些序列的互补序列。下面为代表性的序列伴放线菌素放线杆菌(Actinobacillus actinomycetemcomitans)ssrAGGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCGAAGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCCTTTAGCCTTCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAAGTAGCTTAATTGTCGCGTGTCCGTCAGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO1伴放线菌素放线杆菌(Actinobacillus actinomycetemcomitans)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCGAAGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCCUUUAGCCUUCGCUCCCCAGCUUCCGCUCGUAAGACGGGGAUAAAGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCAUUAAAUUAAAUCAAAGUAGCUUAAUUGUCGCGUGUCCGUCAGCAGGAUUAAGUGAAUUUAAAGACCGGACUAAACGUGUAGUGCUAACGGCAGAGGAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO2杀鲑气单胞菌(Aeromonas salmonicida)ssrA，内部部分AAGATTCACGAAACCCAAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCtTAATAACCTGCATAGAGCCCTTCTACCCTAGCTTGCCTGTGTCCTAGGGAATCGGAAGGTCATCCTTCACAGGATCGTGTGGAAGTCCTGCTCGGGGCGGAAGCATTAAAACCAATCGAGCTAGTCAATTCGTGGCGTGTCTCTCCGCAGCGGGTTGGCGAATGTAAAGAGTGACTAAGCATGTAGTACCGAGGATGTAGTAATTTTGGACGGGG SEQ ID NO3杀鲑气单胞菌(Aeromonas salmonicida)tmRNA，内部部分AAGAUUCACGAAACCCAAGGUGCAUGCCGAGGUGCGGUAGGCCUCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCAUAGAGCCCUUCUACCCUAGCUUGCCUGUGUCCUAGGGAAUCGGAAGGUCAUCCUUCACAGGAUCGUGUGGAAGUCCUGCUCGGGGCGGAAGCAUUAAAACCAAUCGAGCUAGUCAAUUCGUGGCGUGUCUCUCCGCAGCGGGUUGGCGAAUGUAAAGAGUGACUAAGCAUGUAGUACCGAGGAUGUAGUAAUUUUGGACGGGG SEQ ID NO4富养产碱菌(Alcaligenes eutrophus)ssrATGGGCCGACCTGGTTTCGACGTGGTTACAAAGCAGTGAGGCATACCGAGGACCCGTCACCTCGTTAATCAATGGAATGCAATAACTGCTAACGACGAACGTTACGCACTCGCTTAATTGCGGCCGTCCTCGCACTGGCTCGCTGACGGGCTAGGGTCGCAAGACCACGCGAGGTATTTACGTCAGATAAGCTCCGGAAGGGTCACGAAGCCGGGGACGAAAACCTAGTGACTCGCCGTCGTAGAGCGTGTTCGTCCGATGCGCCGGTTAAATCAAATGACAGAACTAAGTATGTAGAACTCTCTGTGGAGGGCTTACGGACGCGGGTTCGATTCCCGCCGGCTCCACCA SEQ IDNO5富养产碱菌(Alcaligenes eutrophus)tmRNAUGGGCCGACCUGGUUUCGACGUGGUUACAAAGCAGUGAGGCAUACCGAGGACCCGUCACCUCGUUAAUCAAUGGAAUGCAAUAACUGCUAACGACGAACGUUACGCACUCGCUUAAUUGCGGCCGUCCUCGCACUGGCUCGCUGACGGGCUAGGGUCGCAAGACCACGCGAGGUAUUUACGUCAGAUAAGCUCCGGAAGGGUCACGAAGCCGGGGACGAAAACCUAGUGACUCGCCGUCGUAGAGCGUGUUCGUCCGAUGCGCCGGUUAAAUCAAAUGACAGAACUAAGUAUGUAGAACUCUCUGUGGAGGGCUUACGGACGCGGGUUCGAUUCCCGCCGGCUCCACCA SEQ ID NO6产液菌(Aquifex aeolicus)ssrAGGGGGCGGAAAGGATTCGACGGGGACAGGCGGTCCCCGAGGAGCAGGCCGGGTGGCTCCCGTAACAGCCGCTAAAACAGCTCCCGAAGCTGAACTCGCTCTCGCTGCCTAATTAAACGGCAGCGCGTCCCCGGTAGGTTTGCGGGTGGCCTACCGGAGGGCGTCAGAGACACCCGCTCGGGCTACTCGGTCGCACGGGGCTGAGTAGCTGACACCTAACCCGTGCTACCCTCGGGGAGCTTGCCCGTGGGCGACCCGAGGGGAAATCCTGAACACGGGCTAAGCCTGTAGAGCCTCGGATGTGGCCGCCGTCCTCGGACGCGGGTTCGATTCCCGCCGCCTCCACCASEQ ID NO7产液菌(Aquifex aeolicus)tmRNAGGGGGCGGAAAGGAUUCGACGGGGACAGGCGGUCCCCGAGGAGCAGGCCGGGUGGCUCCCGUAACAGCCGCUAAAACAGCUCCCGAAGCUGAACUCGCUCUCGCUGCCUAAUUAAACGGCAGCGCGUCCCCGGUAGGUUUGCGGGUGGCCUACCGGAGGGCGUCAGAGACACCCGCUCGGGCUACUCGGUCGCACGGGGCUGAGUAGCUGACACCUAACCCGUGCUACCCUCGGGGAGCUUGCCCGUGGGCGACCCGAGGGGAAAUCCUGAACACGGGCUAAGCCUGUAGAGCCUCGGAUGUGGCCGCCGUCCUCGGACGCGGGUUCGAUUCCCGCCGCCUCCACCA SEQ ID NO8巨大芽孢杆菌(Bacillus megaterium)ssrA，内部部分AGGGTAGTTCGAGCTTAGGTTGCGAGTCGAGGAGATGGCCTCGTTAAAACATCAACGCCAATAATAACTGGCAAATCTAACAATAACTTCGCTTTAGCTGCATAATAGTAGCTTAGCGTTCCTCCCTCCATCGCCCATGTGGTAGGGTAAGGGACTCACTTTAAGTGGGCTACGCCGGAGTTCGCCGTCTGAGGACGAAGGAAGAGAATAATCAGACTAGCGACTGGGACGCCTGTTGGTAGGCAGAACAGCTCGCGAATGATCAATATGCCAACTACACTCGTAGACGCTTAAGTGGCCATATTTCTGGACGTGG SEQ ID NO9巨大芽孢杆菌(Bacillus megaterium)tmRNA，内部部分AGGGUAGUUCGAGCUUAGGUUGCGAGUCGAGGAGAUGGCCUCGUUAAAACAUCAACGCCAAUAAUAACUGGCAAAUCUAACAAUAACUUCGCUUUAGCUGCAUAAUAGUAGCUUAGCGUUCCUCCCUCCAUCGCCCAUGUGGUAGGGUAAGGGACUCACUUUAAGUGGGCUACGCCGGAGUUCGCCGUCUGAGGACGAAGGAAGAGAAUAAUCAGACUAGCGACUGGGACGCCUGUUGGUAGGCAGAACAGCUCGCGAAUGAUCAAUAUGCCAACUACACUCGUAGACGCUUAAGUGGCCAUAUUUCUGGACGUGG SEQ ID NO10枯草芽孢杆菌(Bacillus subtilis)ssrAGGGGACGTTACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACATTGCCTATGTGTCTGTGAAGAGCACATCCAAGTAGGCTACGCTTGCGTTCCCGTCTGAGAACGTAAGAAGAGATGAACAGACTAGCTCTCGGAAGGCCCGCCCGCAGGCAAGAAGATGAGTGAAACCATAAATATGCAGGCTACGCTCGTAGACGCTTAAGTAATCGATGTTTCTGGACGTGGGTTCGACTCCCACCGTCTCCACCA SEQ ID NO11枯草芽孢杆菌(Bacillus subtilis)tmRNAGGGGACGUUACGGAUUCGACAGGGAUGGAUCGAGCUUGAGCUGCGAGCCGAGAGGCGAUCUCGUAAACACGCACUUAAAUAUAACUGGCAAAACUAACAGUUUUAACCAAAACGUAGCAUUAGCUGCCUAAUAAGCGCAGCGAGCUCUUCCUGACAUUGCCUAUGUGUCUGUGAAGAGCACAUCCAAGUAGGCUACGCUUGCGUUCCCGUCUGAGAACGUAAGAAGAGAUGAACAGACUAGCUCUCGGAAGGCCCGCCCGCAGGCAAGAAGAUGAGUGAAACCAUAAAUAUGCAGGCUACGCUCGUAGACGCUUAAGUAAUCGAUGUUUCUGGACGUGGGUUCGACUCCCACCGUCUCCACCA SEQ ID NO12百日咳鲍特氏菌(Bordetella pertussis)ssrAGGGGCCGATCCGGATTCGACGTGGGTCATGAAACAGCTCAGGGCATGCCGAGCACCAGTAAGCTCGTTAATCCACTGGAACACTACAAACGCCAACGACGAGCGTCTCGCTCTCGCCGCTTAAGCGGTGAGCCGCTGCACTGATCTGTCCTTGGGTCAGGCGGGGGAAGGCAACTTCACAGGGGGCAACCCCGAACCGCAGCAGCGACATTCACAAGGAATCGGCCACCGCTGGGGTCACACGGCGTTGGTTTAAATTACGTGAATCGCCCTGGTCCGGCCCGTCGATCGGCTAAGTCCAGGGTTAAATCCAAATAGATCGACTAAGCATGTAGAACTGGTTGCGGAGGGCTTGCGGACGGGGGTTCAATTCCCCCCGGCTCCACCASEQ ID NO13百日咳鲍特氏菌(Bordetella pertussis)tmRNAGGGGCCGAUCCGGAUUCGACGUGGGUCAUGAAACAGCUCAGGGCAUGCCGAGCACCAGUAAGCUCGUUAAUCCACUGGAACACUACAAACGCCAACGACGAGCGUCUCGCUCUCGCCGCUUAAGCGGUGAGCCGCUGCACUGAUCUGUCCUUGGGUCAGGCGGGGGAAGGCAACUUCACAGGGGGCAACCCCGAACCGCAGCAGCGACAUUCACAAGGAAUCGGCCACCGCUGGGGUCACACGGCGUUGGUUUAAAUUACGUGAAUCGCCCUGGUCCGGCCCGUCGAUCGGCUAAGUCCAGGGUUAAAUCCAAAUAGAUCGACUAAGCAUGUAGAACUGGUUGCGGAGGGCUUGCGGACGGGGGUUCAAUUCCCCCCGGCUCCACCASEQ ID NO14布氏疏螺旋体(Borrelia burgdorferi)ssrAGGGGATGTTTTGGATTTGACTGAAAATGTTAATATTGTAAGTTGCAGGCAGAGGGAATCTCTTAAAACTTCTAAAATAAATGCAAAAAATAATAACTTTACAAGCTCAAATCTTGTAATGGCTGCTTAAGTTAGCAGAGGGTTTTGTTGAATTTGGCTTTGAGGTTCACTTATACTCTTTTCGACATCAAAGCTTGCTTAAAAATGTTTTCAAGTTGATTTTTAGGGACTTTTATACTTGAGAGCAATTTGGTGGTTTGCTAGTATTTCCAAACCATATTGCTTAATAAAATACTAGATAAGCTTGTAGAAGCTTATAGTATTATTTTTAGGACGCGGGTTCAATTCCCGCCATCTCCACCA SEQ ID NO15布氏疏螺旋体(Borrelia burgdorferi)tmRNAGGGGAUGUUUUGGAUUUGACUGAAAAUGUUAAUAUUGUAAGUUGCAGGCAGAGGGAAUCUCUUAAAACUUCUAAAAUAAAUGCAAAAAAUAAUAACUUUACAAGCUCAAAUCUUGUAAUGGCUGCUUAAGUUAGCAGAGGGUUUUGUUGAAUUUGGCUUUGAGGUUCACUUAUACUCUUUUCGACAUCAAAGCUUGCUUAAAAAUGUUUUCAAGUUGAUUUUUAGGGACUUUUAUACUUGAGAGCAAUUUGGUGGUUUGCUAGUAUUUCCAAACCAUAUUGCUUAAUAAAAUACUAGAUAAGCUUGUAGAAGCUUAUAGUAUUAUUUUUAGGACGCGGGUUCAAUUCCCGCCAUCUCCACCASEQ ID NO16空肠弯曲杆菌(Campylobacter jejuni)ssrAGGGAGCGACTTGGCTTCGACAGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATTTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAAGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGACAGGGGTTCGATTCCCCTCGCTTCCACCASEQ ID NO17空肠弯曲杆菌(Campylobacter jejuni)tmRNAGGGAGCGACUUGGCUUCGACAGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUUUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAAGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGGACAGGGGUUCGAUUCCCCUCGCUUCCACCA SEQ ID NO18沙眼衣原体(Chlamydia trachomatis(D/UW-3/CX))ssrAGGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTCGAAGATCTAAGAGTAGCTGCTTAATTAGCAAAGTTGTTACCTAAATACGGGTGACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACACCGTCATGTATCTGGTTAGAACTTAGGTCCTTTAATTCTCGAGGAAATGAGTTTGAAATTTAATGAGAGTCGTTAGTCTCTATAGGGGTTTCTAGCTGAGGAGACATAACGTATAGTACCTAGGAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGACTCTCTCCACCTCCACCA SEQ ID NO19沙眼衣原体(Chlamydia trachomatis(D/UW-3/CX))tmRNAGGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUCGAAGAUCUAAGAGUAGCUGCUUAAUUAGCAAAGUUGUUACCUAAAUACGGGUGACCCGGUGUUCGCGAGCUCCACCAGAGGUUUUCGAAACACCGUCAUGUAUCUGGUUAGAACUUAGGUCCUUUAAUUCUCGAGGAAAUGAGUUUGAAAUUUAAUGAGAGUCGUUAGUCUCUAUAGGGGUUUCUAGCUGAGGAGACAUAACGUAUAGUACCUAGGAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACUAAGGACGAGAGUUCGACUCUCUCCACCUCCACCASEQ ID NO20沙眼衣原体(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))ssrAGGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTTAATGATCTAAGAGTTGCTGCTTAATTAGCAAAGTTGTTACCTAAGTACTGGTAACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACGCCGTCATTTATCTGGTTAGAATTAGGGCCTTTTAACTCTCAAGGGAACTAATTTGAATTTTAATGAGAGTCGTTGGTCTCTATAGAGGTTTCTAGCTGAGGAGATATAACGTAAAATATTCTAGAAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGAATCTCTCCACCTCCACCA SEQ ID NO21沙眼衣原体(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))tmRNAGGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUUAAUGAUCUAAGAGUUGCUGCUUAAUUAGCAAAGUUGUUACCUAAGUACUGGUAACCCGGUGUUCGCGAGCUCCACCAGAGGUUUUCGAAACGCCGUCAUUUAUCUGGUUAGAAUUAGGGCCUUUUAACUCUCAAGGGAACUAAUUUGAAUUUUAAUGAGAGUCGUUGGUCUCUAUAGAGGUUUCUAGCUGAGGAGAUAUAACGUAAAAAUUCUAGAAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACUAAGGACGAGAGUUCGAAUCUCUCCACCUCCACCASEQ ID NO22微温杆状绿菌(Chlorobium tepidum)ssrAGGGGATGACAGGCTATCGACAGGATAGGTGTGAGATGTCGTTGCACTCCGAGTTTCAGCATGGACGGACTCGTTAAACAAGTCTATGTACCAATAGATGCAGACGATTATTCGTATGCAATGGCTGCCTGATTAGCACAAGTTAATTCAGAAGCCATCGTCCTGCGGTGAATGCGCTTACTCTGAAGCCGCCGGATGGCATAACCCGCGCTTGAGCCTACGGGTTCGCGCAAGTAAGCTCCGTACATTCATGCCCGAGGGGGTGTGCGGGTAACCAATCGGGATAAGGGGACGAACGCTGCTGGCGGTGTAATCGGACCACGAAAAACCAACCACCAGAGATGAGTGTGGTAACTGCATCGAGCAGTGTCCTGGACGCGGGTTCAAGTCCCGCCATCTCCACCA SEQ ID NO23微温杆状绿菌(Chlorobium tepidum)tmRNAGGGGAUGACAGGCUAUCGACAGGAUAGGUGUGAGAUGUCGUUGCACUCCGAGUUUCAGCAUGGACGGACUCGUUAAACAAGUCUAUGUACCAAUAGAUGCAGACGAUUAUUCGUAUGCAAUGGCUGCCUGAUUAGCACAAGUUAAUUCAGAAGCCAUCGUCCUGCGGUGAAUGCGCUUACUCUGAAGCCGCCGGAUGGCAUAACCCGCGCUUGAGCCUACGGGUUCGCGCAAGUAAGCUCCGUACAUUCAUGCCCGAGGGGGUGUGCGGGUAACCAAUCGGGAUAAGGGGACGAACGCUGCUGGCGGUGUAAUCGGACCACGAAAAACCAACCACCAGAGAUGAGUGUGGUAACUGCAUCGAGCAGUGUCCUGGACGCGGGUUCAAGUCCCGCCAUCUCCACCA SEQ ID NO24Cyanophora paradoxa(藻类)蓝色小体ssrAGGGGCTGTTTAGGTTTCGACGTTTTTTTCTAATTATGTTTGTTAAGCAAGTCGAGGATTTGTTCTATCTCGAAAATCAAGAACTCTCAAAATTTAAACGCAACTAATATTGTACGTTTTAACCGTAAAGCAGCTTTCGCTGTTTAATAATTACTTTTAATTTAAAAACCTAATTTTTTTAGGAATTTATTTATTTATTGTTTATCCTGCTTAATGAATTAAAAAAAGCTATACTTGTGAATAAACGCATAATTTAAAAAAACGGACGTGGGTTCAAATCCCACCAGCTCCACCA SEQ ID NO25Cyanophora paradoxa(藻类)蓝色小体tmRNAGGGGCUGUUUAGGUUUCGACGUUUUUUUCUAAUUAUGUUUGUUAAGCAAGUCGAGGAUUUGUUCUAUCUCGAAAAUCAAGAACUCUCAAAAUUUAAACGCAACUAAUAUUGUACGUUUUAACCGUAAAGCAGCUUUCGCUGUUUAAUAAUUACUUUUAAUUUAAAAACCUAAUUUUUUUAGGAAUUUAUUUAUUUAUUGUUUAUCCUGCUUAAUGAAUUAAAAAAAGCUAUACUUGUGAAUAAACGCAUAAUUUAAAAAAACGGACGUGGGUUCAAAUCCCACCAGCUCCACCASEQ ID NO26丙酮丁醇梭菌(Clostridium acetobutylicum)ssrA，3′部分AATCTGGCGTCGAGAGCGGGGAAACGAGCCTTACAAAGCTTTGAGTAAGGAACGGAATTTATGAAGCTACTGAAGTGAAAAGCTTGTTTGTAGGCGTTTCATGGAGGGAATGTTAAAATACAAACTGCACTCGGAGATGCTTAATGAAACCATTTTCGGACAGGGGTTCGATTCCCCTCGCCTCCACCA SEQ ID NO27丙酮丁醇梭菌(Clostridium acetobutylicum)tmRNA，3′部分AAUCUGGCGUCGAGAGCGGGGAAACGAGCCUUACAAAGCUUUGAGUAAGGAACGGAAUUUAUGAAGCUACUGAAGUGAAAAGCUUGUUUGUAGGCGUUUCAUGGAGGGAAUGUUAAAAUACAAACUGCACUCGGAGAUGCUUAAUGAAACCAUUUUCGGACAGGGGUUCGAUUCCCCUCGCCUCCACCA SEQ ID NO28放射异常球菌模式种(Deinococcus radiodurans)ssrAGGGGGTGACCCGGTTTCGACAGGGGAACTGAAGGTGATGTTGCGTGTCGAGGTGCCGTTGGCCTCGTAAACAAACGGCAAAGCCATTTAACTGGCAACCAGAACTACGCTCTCGCTGCTTAAGTGAGATGACGACCGTGCAGCCCGGCCTTTGGCGTCGCGGAAGTCACTAAAAAAGAAGGCTAGCCCAGGCGATTCTCCATAGCCGACGGCGAAACTTTATGGAGCTACGGCCTGCGAGAACCTGCCCACTGGTGAGCGCCGGCCCGACAATCAAACAGTGGGATACACACGTAGACGCACGCTGGACGGACCTTTGGACGGCGGTTCGACTCCGCCCACCTCCACCASEQ ID NO29放射异常球菌模式种(Deinococcus radiodurans)tmRNAGGGGGUGACCCGGUUUCGACAGGGGAACUGAAGGUGAUGUUGCGUGUCGAGGUGCCGUUGGCCUCGUAAACAAACGGCAAAGCCAUUUAACUGGCAACCAGAACUACGCUCUCGCUGCUUAAGUGAGAUGACGACCGUGCAGCCCGGCCUUUGGCGUCGCGGAAGUCACUAAAAAAGAAGGCUAGCCCAGGCGAUUCUCCAUAGCCGACGGCGAAACUUUAUGGAGCUACGGCCUGCGAGAACCUGCCCACUGGUGAGCGCCGGCCCGACAAUCAAACAGUGGGAUACACACGUAGACGCACGCUGGACGGACCUUUGGACGGCGGUUCGACUCCGCCCACCUCCACCA SEQ ID NO30脱硫脱硫弧菌模式种(Desulfovibrio desulfuricans)ssrA，内部部分GGGACTGGAACCGTAGCGGCAGGTCGAGGCGCCGCTGGCCTCGTAAAAAGCGGCACAAAAGTAATTGCCAACAACGATTACGACTACGCTTACGCTGCCTAATAACAGCGAGGCAATGACCGTTTAACGGTCGCGCCGATCAGGGCCATGCCTGATAACCCTGATTGGCGACACTTATCAGGCTGGCGAAAACCGGCTCTCGCCGGGGTTTTTCGCGAGGAGTTTACCGGCGGGATTGCTGCGTTGTGCCTGGTCAGGGGCCAACAGCGCGGTGAAATACATACTTGACCTAAACCTGTAATGCTTCGTGTGGAATGTTCTCGGACGGGG SEQ ID NO31脱硫脱硫弧菌模式种(Desulfovibrio desulfuricans)tmRNA，内部部分GGGACUGGAACCGUAGCGGCAGGUCGAGGCGCCGCUGGCCUCGUAAAAAGCGGCACAAAAGUAAUUGCCAACAACGAUUACGACUACGCUUACGCUGCCUAAUAACAGCGAGGCAAUGACCGUUUAACGGUCGCGCCGAUCAGGGCCAUGCCUGAUAACCCUGAUUGGCGACACUUAUCAGGCUGGCGAAAACCGGCUCUCGCCGGGGUUUUUCGCGAGGAGUUUACCGGCGGGAUUGCUGCGUUGUGCCUGGUCAGGGGCCAACAGCGCGGUGAAAUACAUACUUGACCUAAACCUGUAAUGCUUCGUGUGGAAUGUUCUCGGACGGGG SEQ ID NO32节瘤腐蹄杆菌(Dichelobacter nodosus)ssrA，3′部分CTCGAGGTGCATGTCGAGAATGAGAGAATCTCGTTAAATACTTTCAAAACTTATAGTTGCAAACGACGACAACTACGCTTTAGCGGCTTAATTCCCGCTTTCGCTTACCTAGATTTGTCTGTGGGTTTACCGTAAGCGACATTAACACAGAATCGCTGGTTAACGCGTCCGCTGTTAATCGGTTAAATTAAGCGGAATCGCTTGTAAAATGCCTGAGCGTTGGCTGTTTATGAGTTAAACCTAATTAACTGCTCTAAACATGTAGTACCAAAAGTTAAGGATTCGCGGACGGGGGTTCAAATCCCCCCGCCTCCACCA SEQ ID NO33节瘤腐蹄杆菌(Dichelobacter nodosus)tmRNA，3′部分CUCGAGGUGCAUGUCGAGAAUGAGAGAAUCUCGUUAAAUACUUUCAAAACUUAUAGUUGCAAACGACGACAACUACGCUUUAGCGGCUUAAUUCCCGCUUUCGCUUACCUAGAUUUGUCUGUGGGUUUACCGUAAGCGACAUUAACACAGAAUCGCUGGUUAACGCGUCCGCUGUUAAUCGGUUAAAUUAAGCGGAAUCGCUUGUAAAAUGCCUGAGCGUUGGCUGUUUAUGAGUUAAACCUAAUUAACUGCUCUAAACAUGUAGUACCAAAAGUUAAGGAUUCGCGGACGGGGGUUCAAAUCCCCCCGCCUCCACCA SEQ ID NO34肠球菌模式种(Enterococcus faecalis)ssrAGGGGGCGTTACGGATTCGACAGGCATAGTTGAGCTTGAATTGCGTTTCGTAGGTTACGGCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAATTCTTTCGCTTTAGCTGCCTAAAAACCAGCTAGCGAAGATCCTCCCGGCATCGCCCATGTGCTCGGGTCAGGGTCCTAATCGAAGTGGGATACGCTAAATTTTTCCGTCTGTAAAATTTAGAGGAGCTTACCAGACTAGCAATACAGAATGCCTGTCACTCGGCACGCTGTAAAGCGAACCTTTAAATGAGTGTCTATGAACGTAGAGATTTAAGTGGCAATATGTTTGGACGCGGGTTCGACTCCCGCCGTCTCCACCA SEQ ID NO35肠球菌模式种(Enterococcus faecalis)tmRNAGGGGGCGUUACGGAUUCGACAGGCAUAGUUGAGCUUGAAUUGCGUUUCGUAGGUUACGGCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAAUUCUUUCGCUUUAGCUGCCUAAAAACCAGCUAGCGAAGAUCCUCCCGGCAUCGCCCAUGUGCUCGGGUCAGGGUCCUAAUCGAAGUGGGAUACGCUAAAUUUUUCCGUCUGUAAAAUUUAGAGGAGCUUACCAGACUAGCAAUACAGAAUGCCUGUCACUCGGCACGCUGUAAAGCGAACCUUUAAAUGAGUGUCUAUGAACGUAGAGAUUUAAGUGGCAAUAUGUUUGGACGCGGGUUCGACUCCCGCCGUCUCCACCA SEQ ID NO36大肠杆菌(Escherichia coli)ssrAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA SEQ ID NO37大肠杆菌(Escherichia coli)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUUAAUCAGGCUAGUUUGUUAGUGGCGUGUCCGUCCGCAGCUGGCAAGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA SEQ ID NO38流感嗜血杆菌(Haemophilus influenzae)ssrAGGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCAAGGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCATTTAGCCTTCGCGCTCCAGCTTCCGCTCGTAAGACGGGGATAACGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCACTAAATTGAATCAAACTAGCTTAAGTTTAGCGTGTCTGTCCGCATGCTTAAGTGAAATTAAAGACGAGACTAAACGTGTAGTACTGAAGGTAGAGTAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO39流感嗜血杆菌(Haemophilus influenzae)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCAAGGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCAUUUAGCCUUCGCGCUCCAGCUUCCGCUCGUAAGACGGGGAUAACGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCACUAAAUUGAAUCAAACUAGCUUAAGUUUAGCGUGUCUGUCCGCAUGCUUAAGUGAAAUUAAAGACGAGACUAAACGUGUAGUACUGAAGGUAGAGUAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO40幽门螺杆菌(Helicobacter pylori)(ATCC 43504)ssrA，内部部分AGATTTCTTGTCGCGCAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTTAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAACTAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGG SEQ ID NO41幽门螺杆菌(Helicobacter pylori)(ATCC 43504)tmRNA，内部部分AGAUUUCUUGUCGCGCAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUUAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAACUAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGSEQ ID NO42幽门螺杆菌(Helicobacter pylori)(菌株26695)ssrAGGGGCTGACTTGGATTTCGACAGATTTCTTGTCGCACAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTCAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAAACCAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGGGTTCGATTCCCCACAGCTCCACCA SEQ ID NO43幽门螺杆菌(Helicobacter pylori)(菌株26695)tmRNAGGGGCUGACUUGGAUUUCGACAGAUUUCUUGUCGCACAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUCAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAAACCAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGGUUCGAUUCCCCACAGCUCCACCASEQ ID NO44产气克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)ssrA，内部部分GGGATTCGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTAAGAGCCCTCTCTCCCTAGCTTCCGCTCCTAAGACGGGGAATAAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTAATCAGGCTAGTTTGTCAGTGGCGTGTCCGTCCGCAGCTGGCCAGCGAATGTAAAGACTGGACTAAGCATGTAGTGCCGAGGATGTAGGAATTTC SEQ ID NO45产气克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)tmRNA，内部部分GGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUAAGAGCCCUCUCUCCCUAGCUUCCGCUCCUAAGACGGGGAAUAAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUAAUCAGGCUAGUUUGUCAGUGGCGUGUCCGUCCGCAGCUGGCCAGCGAAUGUAAAGACUGGACUAAGCAUGUAGUGCCGAGGAUGUAGGAAUUUC SEQ ID NO46乳杆菌模式种(Lactobacillus lactis)(NCTC 662)ssrA，内部部分AAGCACAGTTCGAGCTTGAATTGCGTTTCGTAGGTTACGTCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAACTCTTACGCTTTAGCTGCCTAAAAACAGTTAGCGTAGATCCTCTCGGCATCGCCCATGTGCTCGAGTAAGGGTCTCAAATTTAGTGGGATACGTTAAACTTTTCCGTCTGTAAAGTTTAAAAGAGATCATCAGACTAGCGATACAGAATGCCTGTCACTCGGCAAGCTGTAAAGCGAAACCTCAAATGAGTTGACTATGAACGTAGATTTTTAAGTGTCGATGTGTTT SEQ ID NO47乳杆菌模式种(Lactobacillus lactis)(NCTC 662)tmRNA，内部部分AAGCACAGUUCGAGCUUGAAUUGCGUUUCGUAGGUUACGUCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAACUCUUACGCUUUAGCUGCCUAAAAACAGUUAGCGUAGAUCCUCUCGGCAUCGCCCAUGUGCUCGAGUAAGGGUCUCAAAUUUAGUGGGAUACGUUAAACUUUUCCGUCUGUAAAGUUUAAAAGAGAUCAUCAGACUAGCGAUACAGAAUGCCUGUCACUCGGCAAGCUGUAAAGCGAAACCUCAAAUGAGUUGACUAUGAACGUAGAUUUUUAAGUGUCGAUGUGUUU SEQ ID NO48嗜肺军团菌模式种(Legionella pneumophila)ssrA，内部部分GTGGGTTGCAAAACCGGAAGTGCATGCCGAGAAGGAGATCTCTCGTAAATAAGACTCAATTAAATATAAATGCAAACGATGAAAACTTTGCTGGTGGGGAAGCTATCGCTGCCTAATAAGCACTTTAGTTAAACCATCACTGTGTACTGGCCAATAAACCCAGTATCCCGTTCGACCGAGCCCGCTTATCGGTATCGAATCAACGGTCATAAGAGATAAGCTAGCGTCCTAATCTATCCCGGGTTATGGCGCGAAACTCAGGGAATCGCTGTGTATCATCCTGCCCGTCGGAGGAGCCACAGTTAAATTCAAAAGACAAGGCTATGCATGTAGAGCTAAAGGCAGAGGACTTGCGGACGCGG SEQ ID NO49嗜肺军团菌模式种(Legionella pneumophila)tmRNA，内部部分GUGGGUUGCAAAACCGGAAGUGCAUGCCGAGAAGGAGAUCUCUCGUAAAUAAGACUCAAUUAAAUAUAAAUGCAAACGAUGAAAACUUUGCUGGUGGGGAAGCUAUCGCUGCCUAAUAAGCACUUUAGUUAAACCAUCACUGUGUACUGGCCAAUAAACCCAGUAUCCCGUUCGACCGAGCCCGCUUAUCGGUAUCGAAUCAACGGUCAUAAGAGAUAAGCUAGCGUCCUAAUCUAUCCCGGGUUAUGGCGCGAAACUCAGGGAAUCGCUGUGUAUCAUCCUGCCCGUCGGAGGAGCCACAGUUAAAUUCAAAAGACAAGGCUAUGCAUGUAGAGCUAAAGGCAGAGGACUUGCGGACGCGG SEQ ID NO50格氏李斯特氏菌(Listeria grayi)ssrA，内部部分ACAGGGATAGGTCGAGCTTGAGTTGCGAGCCGGGGGGATCGGCCCGTCATCAACGTCAAAGCCAATAATAACTGGCAAACAAAACAACAATTTAGCTTTCGCTGCCTAATAGCAGTCTGAATAGCTGATCCTCCGTGCATCACCCATGTGCTACGGTAAGGGTCTCACTTTTAAGTGGGTTACGCTGGCTTATCTCCGTCTGGGGCAAACGAGAAGAGCATAATCAGACTAGCTAGATAGAGCCCTGACGCCGGGCAGACATCTATGCGAAATCCAAATACGGCAACTACGCTCGTAGATGCTCAAGTGCCGATATTTCTGG SEQ ID NO51格氏李斯特氏菌(Listeria grayi)tmRNA，内部部分ACAGGGAUAGGUCGAGCUUGAGUUGCGAGCCGGGGGGAUCGGCCCGUCAUCAACGUCAAAGCCAAUAAUAACUGGCAAACAAAACAACAAUUUAGCUUUCGCUGCCUAAUAGCAGUCUGAAUAGCUGAUCCUCCGUGCAUCACCCAUGUGCUACGGUAAGGGUCUCACUUUUAAGUGGGUUACGCUGGCUUAUCUCCGUCUGGGGCAAACGAGAAGAGCAUAAUCAGACUAGCUAGAUAGAGCCCUGACGCCGGGCAGACAUCUAUGCGAAAUCCAAAUACGGCAACUACGCUCGUAGAUGCUCAAGUGCCGAUAUUUCUGG SEQ ID NO52无害李斯特氏菌(Listeria innocua)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG SEQ ID NO53无害李斯特氏菌(Listeria innocua)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG SEQ ID NO54单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 7973)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO55单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC7973)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO56单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)ssrA，内部部分CAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTT SEQ ID NO57单核细胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)tmRNA，内部部分CAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUSEQ ID NO58墨氏李斯特氏菌(Listeria murrayi)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATGAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG SEQ ID NO59墨氏李斯特氏菌(Listeria murrayi)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUGAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG SEQ ID NO60威氏李斯特氏菌(Listeria welshimeri)ssrA，内部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTGGCTAATCTCCGTCTGAGGTTAGTTGGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO61威氏李斯特氏菌(Listeria welshimeri)tmRNA，内部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUGGCUAAUCUCCGUCUGAGGUUAGUUGGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO62除烃海杆菌(Marinobacter hydrocarbonoclasticus)ssrA，内部部分GCCGGTGACGAACCCTTGGGTGCATGCCGAGATGGCAGCGAATCTCGTAAATCCAAAGCTGCAACGTAATAGTCGCAAACGACGAAAACTACGCACTGGCGGCGTAAGCCGTTCCAGTCGTCCTGGCTGAGGCGCCTATAACTCAGTAGCAACATCCCAGGACGTCATCGCTTATAGGCTGCTCCGTTCACCAGAGCTCACTGGTGTTCGGCTAAGATTAAAGAGCTCGCCTCTTGCACCCTGACCTTCGGGTCGCTTGAGGTTAAATCAATAGAAGGACACTAAGCATGTAGACCTCAAGGCCTAGTGCTGGCGGACGCGG SEQ ID NO63除烃海杆菌(Marinobacter hydrocarbonoclasticus)tmRNA，内部部分GCCGGUGACGAACCCUUGGGUGCAUGCCGAGAUGGCAGCGAAUCUCGUAAAUCCAAAGCUGCAACGUAAUAGUCGCAAACGACGAAAACUACGCACUGGCGGCGUAAGCCGUUCCAGUCGUCCUGGCUGAGGCGCCUAUAACUCAGUAGCAACAUCCCAGGACGUCAUCGCUUAUAGGCUGCUCCGUUCACCAGAGCUCACUGGUGUUCGGCUAAGAUUAAAGAGCUCGCCUCUUGCACCCUGACCUUCGGGUCGCUUGAGGUUAAAUCAAUAGAAGGACACUAAGCAUGUAGACCUCAAGGCCUAGUGCUGGCGGACGCGG SEQ ID NO64鸟分枝杆菌(Mycobacterium avium)ssrA，内部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA SEQ ID NO65鸟分枝杆菌(Mycobacterium avium)tmRNA，内部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO66牛分枝杆菌(Mycobacterium bovis)ssrA，内部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGG SEQ ID NO67牛分枝杆菌(Mycobacterium bovis)tmRNA，内部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGG SEQ ID NO68麻风分枝杆菌(Mycobacterium leprae)ssrAGGGGCTGAAAGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAGCAATATAAGCGCCGATTCATATCAGCGCGACTATGCTCTCGCTGCCTAAGCGATGGCTAGTCTGTCAGACCGGGAACGCCCTCGTCCCGGAGCCTGGCATCAGCTAGAGGGATCTACCGATGGGTTCGGTCGCGGGACTCGTCGGGACACCAACCGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGATCAGGAGATCCGAGTAGAGGCATAGCGAACTACGCACGGAGAAGCCTTGAGGGAAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA SEQ ID NO69麻风分枝杆菌(Mycobacterium leprae)tmRNAGGGGCUGAAAGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAGCAAUAUAAGCGCCGAUUCAUAUCAGCGCGACUAUGCUCUCGCUGCCUAAGCGAUGGCUAGUCUGUCAGACCGGGAACGCCCUCGUCCCGGAGCCUGGCAUCAGCUAGAGGGAUCUACCGAUGGGUUCGGUCGCGGGACUCGUCGGGACACCAACCGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGAUCAGGAGAUCCGAGUAGAGGCAUAGCGAACUACGCACGGAGAAGCCUUGAGGGAAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA SEQ ID NO70鸟分枝杆菌(副结核亚种)(Mycobacterium paratuberculosis)ssrA，内部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA SEQ ID NO71鸟分枝杆菌(副结核亚种)(Mycobacterium paratuberculosis)tmRNA，内部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO72结核分枝杆菌(Mycobacterium tuberculosis)ssrAGGGGCTGAACGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA SEQ ID NO73结核分枝杆菌(Mycobacterium tuberculosis)tmRNAGGGGCUGAACGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA SEQ ID NO74山羊枝原体(Mycoplasma capricolum)ssrAGGGGATGTCATGGATTTGACAGGATATCTTTAGTACATATAAGCAGTAGTGTTGTAGACTATAAATACTACTAGGTTTAAAAAAACGCAAATAAAAACGAAGAAACTTTTGAAATGCCAGCATTTATGATGAATAATGCATCAGCTGGAGCAAACTTTATGTTTGCTTAATAACTACTAGTTTAGTTATAGTATTTCACGAATTATAGATATTTTAAGCTTTATTTATAACCGTATTACCCAAGCTTAATAGAATATATGATTGCAATAAATATATTTGAAATCTAATTGCAAATGATATTTAACCTTTAGTTAATTTTAGTTAAATATTTTAATTAGAAAATTAACTAAACTGTAGAAAGTATGTATTAATATATCTTGGACGCGAGTTCGATTCTCGCCATCTCCACCA SEQ ID NO75山羊枝原体(Mycoplasma capricolum)tmRNAGGGGAUGUCAUGGAUUUGACAGGAUAUCUUUAGUACAUAUAAGCAGUAGUGUUGUAGACUAUAAAUACUACUAGGUUUAAAAAAACGCAAAUAAAAACGAAGAAACUUUUGAAAUGCCAGCAUUUAUGAUGAAUAAUGCAUCAGCUGGAGCAAACUUUAUGUUUGCUUAAUAACUACUAGUUUAGUUAUAGUAUUUCACGAAUUAUAGAUAUUUUAAGCUUUAUUUAUAACCGUAUUACCCAAGCUUAAUAGAAUAUAUGAUUGCAAUAAAUAUAUUUGAAAUCUAAUUGCAAAUGAUAUUUAACCUUUAGUUAAUUUUAGUUAAAUAUUUUAAUUAGAAAAUUAACUAAACUGUAGAAAGUAUGUAUUAAUAUAUCUUGGACGCGAGUUCGAUUCUCGCCAUCUCCACCA SEQ ID NO76生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#1)ssrAGGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGAACCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA SEQ ID NO77生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#1)tmRNAGGGGAUGUUUUGGGUUUGACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAACCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA SEQ ID NO78生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，内部部分ACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCSEQ ID NO79生殖道枝原体(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，内部部分ACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAUCCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUC SEQ ID NO80嗜肺枝原体(Mycoplasma pneumophila)ssrAGGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTTGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA SEQ ID NO81嗜肺枝原体(Mycoplasma pneumophila)tmRNAGGGGAUGUAGAGGUUUUGACAUAAUGUUGAAAGGAAAACAGUUGCAGUGGGGUAUGCCCCUUACAGCUCUAGGUAUAAUAACCGACAAAAAUAACGACGAAGUUUUGGUAGAUCCAAUGUUGAUCGCUAACCAACAAGCAAGUAUCAACUACGCUUUCGCUUAGAACAUACUAAAGCUACACGAAUUGAAUCGCCAUAGUUUGGUUCGUGUCACAGUUUAUGGCUCGGGGUUAACUGGUUCAACUUAAUCCUUAAAUUAUGAACUUAUCGUUUACUUGUUUGUCUUAUGAUCUAAAGUAAGCGAGACAUUAAAACAUAAGACUAAACUGUAGAAGCUGUUUUACCAAUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA SEQ ID NO82淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)ssrA，内部部分GGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGG SEQ ID NO83淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)tmRNA，内部部分GGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGG SEQ ID NO84淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)ssrAGGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGTGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA SEQ ID NO85淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)tmRNAGGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGUGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA SEQ ID NO86脑膜炎奈瑟氏球菌(Neisseria meningitidis)ssrAGGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCTGCCGGGTTATTTGGCAGGAAATGAGATTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA SEQ ID NO87脑膜炎奈瑟氏球菌(Neisseria meningitidis)tmRNAGGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCUGCCGGGUUAUUUGGCAGGAAAUGAGAUUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA SEQ ID NO88Nostoc muscorum PCC7120 ssrAGGGTCCGTCGGTTTCGACAGGTTGGCGAACGCTACTCTGTGATTCAGGTCGAGAGTGAGTCTCCTCTGCAAATCAAGGCTCAAAACAAAAGTAAATGCGAATAACATCGTTAAATTTGCTCGTAAGGACGCTCTAGTAGCTGCCTAAATAGCCTCTTTCAGGTTCGAGCGTCTTCGGTTTGACTCCGTTAAGGACTGAAGACCAACCCCCAACGGATGCTCTAGCAATGTTCTCTGGTTGGCTTGCTAGCTAAGATTTAATCAGAGCATCCTACGTTCGGGATAATGAACGATTCCCGCCTTGAGGGTCAGAAAGGCTAAACCTGTGAATGAGCGGGGGGTCAATACCCAATTTGGACAGCAGTTCGACTCTGCTCGATCCACCA SEQ ID NO89Nostoc muscorum PCC7120 tmRNAGGGUCCGUCGGUUUCGACAGGUUGGCGAACGCUACUCUGUGAUUCAGGUCGAGAGUGAGUCUCCUCUGCAAAUCAAGGCUCAAAACAAAAGUAAAUGCGAAUAACAUCGUUAAAUUUGCUCGUAAGGACGCUCUAGUAGCUGCCUAAAUAGCCUCUUUCAGGUUCGAGCGUCUUCGGUUUGACUCCGUUAAGGACUGAAGACCAACCCCCAACGGAUGCUCUAGCAAUGUUCUCUGGUUGGCUUGCUAGCUAAGAUUUAAUCAGAGCAUCCUACGUUCGGGAUAAUGAACGAUUCCCGCCUUGAGGGUCAGAAAGGCUAAACCUGUGAAUGAGCGGGGGGUCAAUACCCAAUUUGGACAGCAGUUCGACUCUGCUCGAUCCACCASEQ ID NO90Odontella sinensis(硅藻属)叶绿体ssrAGGGGCTGACTTGGTTTCGACATTTAAAAATTGTTACAGTATGATGCAGGTCGAAGTTTCTAATCTTCGTAAAAAAAGAGAAATTTATAATAAATGCTAATAATTTAATTTCTTCTGTGTTTAAAAGTTTATCAACTAAGCAAAATAGTTTAAATTTAAGTTTTGCTGTTTAAGTTTTATGCACATTTAATGATCTAGTAAATAACTTTGTTCGCTATAATTTATATTTATAACTAGACTTTTGTCTTTTTTATAGTTTAGAATAACTTTATCATTTCAAACCTCGTTCCATCTAGTTGAACTAAACCTGTGAACGAATACTATAATAAAATTTTTAGATGGACGTGGGTTCGACTCCCATCAGCTCCACCA SEQ ID NO91Odontella sinensis(硅藻属)叶绿体tmRNAGGGGCUGACUUGGUUUCGACAUUUAAAAAUUGUUACAGUAUGAUGCAGGUCGAAGUUUCUAAUCUUCGUAAAAAAAGAGAAAUUUAUAAUAAAUGCUAAUAAUUUAAUUUCUUCUGUGUUUAAAAGUUUAUCAACUAAGCAAAAUAGUUUAAAUUUAAGUUUUGCUGUUUAAGUUUUAUGCACAUUUAAUGAUCUAGUAAAUAACUUUGUUCGCUAUAAUUUAUAUUUAUAACUAGACUUUUGUCUUUUUUAUAGUUUAGAAUAACUUUAUCAUUUCAAACCUCGUUCCAUCUAGUUGAACUAAACCUGUGAACGAAUACUAUAAUAAAAUUUUUAGAUGGACGUGGGUUCGACUCCCAUCAGCUCCACCASEQ ID NO92卟啉单胞菌(Porphyra purpureum)(红藻)叶绿体ssrAGGGGCTGCAAGGTTTCTACATTGTGAAAAAACAAATATATGAAAGTAAAACGAGCTCATTATTAGAGCTTTTAGTTAAATAAATGCAGAAAATAATATTATTGCTTTTTCTCGAAAATTAGCTGTTGCATAAATAGTCTCAATTTTTGTAATTCGAAGTGATAGACTCTTATACACTACGAATATTCTGTTAGAGTTGCTCTTAATAAAAGAAAAGTAAAAAAATACAAATTCTTATGTTTTTTACCTGAATTGATTCAATTTAAGGTTAGTATTTTTTGATTTTTACAATGGACGTGGGTTCAAGTCCCACCAGCTCCACCA SEQ ID NO93卟啉单胞菌(Porphyra purpureum)(红藻)叶绿体tmRNAGGGGCUGCAAGGUUUCUACAUUGUGAAAAAACAAAUAUAUGAAAGUAAAACGAGCUCAUUAUUAGAGCUUUUAGUUAAAUAAAUGCAGAAAAUAAUAUUAUUGCUUUUUCUCGAAAAUUAGCUGUUGCAUAAAUAGUCUCAAUUUUUGUAAUUCGAAGUGAUAGACUCUUAUACACUACGAAUAUUCUGUUAGAGUUGCUCUUAAUAAAAGAAAAGUAAAAAAAUACAAAUUCUUAUGUUUUUUACCUGAAUUGAUUCAAUUUAAGGUUAGUAUUUUUUGAUUUUUACAAUGGACGUGGGUUCAAGUCCCACCAGCUCCACCA SEQ ID NO94牙龈卟啉单胞菌(Porphyromonas gingivalis)ssrAGGGGCTGACCGGCTTTGACAGCGTGATGAAGCGGTATGTAAGCATGTAGTGCGTGGGTGGCTTGCACTATAATCTCAGACATCAAAAGTTTAATTGGCGAAAATAACTACGCTCTCGCTGCGTAATCGAAGAATAGTAGATTAGACGCTTCATCGCCGCCAAAGTGGCAGCGACGAGACATCGCCCGAGCAGCTTTTTCCCGAAGTAGCTCGATGGTGCGGTGCTGACAAATCGGGAACCGCTACAGGATGCTTCCTGCCTGTGGTCAGATCGAACGGAAGATAAGGATCGTGCATTGGGTCGTTTCAGCCTCCGCTCGCTCACGAAAATTCCAACTGAAACTAAACATGTAGAAAGCATATTGATTCCATGTTTGGACGAGGGTTCAATTCCCTCCAGCTCCACCA SEQ ID NO95牙龈卟啉单胞菌(Porphyromonas gingivalis)tmRNAGGGGCUGACCGGCUUUGACAGCGUGAUGAAGCGGUAUGUAAGCAUGUAGUGCGUGGGUGGCUUGCACUAUAAUCUCAGACAUCAAAAGUUUAAUUGGCGAAAAUAACUACGCUCUCGCUGCGUAAUCGAAGAAUAGUAGAUUAGACGCUUCAUCGCCGCCAAAGUGGCAGCGACGAGACAUCGCCCGAGCAGCUUUUUCCCGAAGUAGCUCGAUGGUGCGGUGCUGACAAAUCGGGAACCGCUACAGGAUGCUUCCUGCCUGUGGUCAGAUCGAACGGAAGAUAAGGAUCGUGCAUUGGGUCGUUUCAGCCUCCGCUCGCUCACGAAAAUUCCAACUGAAACUAAACAUGUAGAAAGCAUAUUGAUUCCAUGUUUGGACGAGGGUUCAAUUCCCUCCAGCUCCACCA SEQ ID NO96雷氏变性菌(Proteus rettgeri)ssrA(NCTC 10975)，内部部分GGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCTTGCCTGGGGTTGAAGCGTTAAACTTAATCAGGATAGTTTGTTGGTGGCGTGTCTGTCCGCAGCTGGCAAATGAATTCAAAGACTAGACTAAGCATGTAGTACCGAGGATGTAGAAATTTCSEQ ID NO97雷氏变性菌(Proteus rettgeri)ssrA tmRNA(NCTC 10975)，内部部分GGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCUUGCCUGGGGUUGAAGCGUUAAACUUAAUCAGGAUAGUUUGUUGGUGGCGUGUCUGUCCGCAGCUGGCAAAUGAAUUCAAAGACUAGACUAAGCAUGUAGUACCGAGGAUGUAGAAAUUUC SEQ ID NO98游海假交替单胞菌(Pseudoalteromonas haloplanktoni)ssrA，内部部分GGAATTCAAGAAGCCCGAGGTGCATGTCGAGGTGCGGTTTGCCTCGTAAAAAAGCCGCAATTTAAAGTAATCGCAAACGACGATAACTACTCTCTAGCAGCTTAGGCTGGCTAGCGCTCCTTCCATGTATTCTTGTGGACTGGATTTTGGAGTGTCACCCTAACACCTGATCGCGACGGAAACCCTGGCCGGGGTTGAAGCGTTAAAACTAAGCGGCCTCGCCTTTATCTACCGTGTTTGTCCGGGATTTAAAGGTTAATTAAATGACAATACTAAACATGTAGTACCGACGGTCGAGGCTTTTCGGACGGGG SEQ ID NO99游海假交替单胞菌(Pseudoalteromonas haloplanktoni)tmRNA，内部部分GGAAUUCAAGAAGCCCGAGGUGCAUGUCGAGGUGCGGUUUGCCUCGUAAAAAAGCCGCAAUUUAAAGUAAUCGCAAACGACGAUAACUACUCUCUAGCAGCUUAGGCUGGCUAGCGCUCCUUCCAUGUAUUCUUGUGGACUGGAUUUUGGAGUGUCACCCUAACACCUGAUCGCGACGGAAACCCUGGCCGGGGUUGAAGCGUUAAAACUAAGCGGCCUCGCCUUUAUCUACCGUGUUUGUCCGGGAUUUAAAGGUUAAUUAAAUGACAAUACUAAACAUGUAGUACCGACGGUCGAGGCUUUUCGGACGGGG SEQ ID NO100铜绿假单胞菌(Pseudomonas aeruginosa)ssrAGGGGCCGATTAGGATTCGACGCCGGTAACAAAAGTTGAGGGGCATGCCGAGTTGGTAGCAGAACTCGTAAATTCGCTGCTGCAAACTTATAGTTGCCAACGACGACAACTACGCTCTAGCTGCTTAATGCGGCTAGCAGTCGCTAGGGGATGCCTGTAAACCCGAAACGACTGTCAGATAGAACAGGATCGCCGCCAAGTTCGCTGTAGACGTAACGGCTAAAACTCATACAGCTCGCTCCAAGCACCCTGCCACTCGGGCGGCGCGGAGTTAACTCAGTAGAGCTGGCTAAGCATGTAAAACCGATAGCGGAAAGCTGGCGGACGGGGGTTCAAATCCCCCCGGTTCCACCASEQ ID NO101铜绿假单胞菌(Pseudomonas aeruginosa)tmRNAGGGGCCGAUUAGGAUUCGACGCCGGUAACAAAAGUUGAGGGGCAUGCCGAGUUGGUAGCAGAACUCGUAAAUUCGCUGCUGCAAACUUAUAGUUGCCAACGACGACAACUACGCUCUAGCUGCUUAAUGCGGCUAGCAGUCGCUAGGGGAUGCCUGUAAACCCGAAACGACUGUCAGAUAGAACAGGAUCGCCGCCAAGUUCGCUGUAGACGUAACGGCUAAAACUCAUACAGCUCGCUCCAAGCACCCUGCCACUCGGGCGGCGCGGAGUUAACUCAGUAGAGCUGGCUAAGCAUGUAAAACCGAUAGCGGAAAGCUGGCGGACGGGGGUUCAAAUCCCCCCGGUUCCACCA SEQ ID NO102鼠伤寒沙门氏菌(Salmonella typhimurium)ssrAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGCGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA SEQ ID NO103鼠伤寒沙门氏菌(Salmonella typhimurium)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGCGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA SEQ ID NO104希瓦氏菌模式种(Shewanella putrefaciens)ssrAGGGGGCGATTCTGGATTCGACAGGATTCACGAAACCCTGGGAGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAGTTATAGTTGCAAACGACGATAACTACGCTCTAGCCGCTTAATGCCGCTAGCCATCTACCACACGCTTTGCACATGGGCAGTGGATTTGATGGTCATCTCACATCGTGCTAGCGAGGGAACCCTGTCTGGGGGTGAACCGCGAAACAGTACCGGACTCACCGTGTGGGATCCTGTCTTTCGGAGTTCAAACGGTTAAACAATAGAAAGACTAAGCATGTAGCGCCTTGGATGTAGGTTTTCTGGACGCGGGTTCAAGTCCCGCCGCCTCCACCASEQ ID NO105希瓦氏菌模式种(Shewanella putrefaciens)tmRNAGGGGGCGAUUCUGGAUUCGACAGGAUUCACGAAACCCUGGGAGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAGUUAUAGUUGCAAACGACGAUAACUACGCUCUAGCCGCUUAAUGCCGCUAGCCAUCUACCACACGCUUUGCACAUGGGCAGUGGAUUUGAUGGUCAUCUCACAUCGUGCUAGCGAGGGAACCCUGUCUGGGGGUGAACCGCGAAACAGUACCGGACUCACCGUGUGGGAUCCUGUCUUUCGGAGUUCAAACGGUUAAACAAUAGAAAGACUAAGCAUGUAGCGCCUUGGAUGUAGGUUUUCUGGACGCGGGUUCAAGUCCCGCCGCCUCCACCA SEQ ID NO106金黄色葡萄球菌(Staphylococcus aureus)ssrAGGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTCGGAGGGTTGTCTTCGTCATCAACACACACAGTTTATAATAACTGGCAAATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCATTTCCTATGTGCTGTTAACGCGATTCAACCTTAATAGGATATGCTAAACACTGCCGTTTGAAGTCTGTTTAGAAGAAACTTAATCAAACTAGCATCATGTTGGTTGTTTATCACTTTTCATGATGCGAAACCTATCGATAAACTACACACGTAGAAAGATGTGTATCAGGACCTTTGGACGCGGGTTCAAATCCCGCCGTCTCCACCA SEQ ID NO107金黄色葡萄球菌(Staphylococcus aureus)tmRNAGGGGACGUUCAUGGAUUCGACAGGGGUCCCCCGAGCUCAUUAAGCGUGUCGGAGGGUUGUCUUCGUCAUCAACACACACAGUUUAUAAUAACUGGCAAAUCAAACAAUAAUUUCGCAGUAGCUGCCUAAUCGCACUCUGCAUCGCCUAACAGCAUUUCCUAUGUGCUGUUAACGCGAUUCAACCUUAAUAGGAUAUGCUAAACACUGCCGUUUGAAGUCUGUUUAGAAGAAACUUAAUCAAACUAGCAUCAUGUUGGUUGUUUAUCACUUUUCAUGAUGCGAAACCUAUCGAUAAACUACACACGUAGAAAGAUGUGUAUCAGGACCUUUGGACGCGGGUUCAAAUCCCGCCGUCUCCACCA SEQ ID NO108戈氏链球菌(Streptococcus gordonii)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATAATACTTCTTACGCTTTAGCTGCCTAAAAACCAGCGGGCGTGACCCGATTCGGATTGCTTGTGTCTGATGACAGGTCTTATTATTAGCAAGCTACGGTAGAATCTTGTCTAGTGATTTTACAAGAGATTGATAGACTCGCTTGATTTGGGCTTGAGTTATGTGTCAAAATCAAGTTAAAACAATACATAGCCTATGGTTGTAGACAAATGTGTTGGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCA SEQ ID NO109戈氏链球菌(Streptococcus gordonii)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUAAUACUUCUUACGCUUUAGCUGCCUAAAAACCAGCGGGCGUGACCCGAUUCGGAUUGCUUGUGUCUGAUGACAGGUCUUAUUAUUAGCAAGCUACGGUAGAAUCUUGUCUAGUGAUUUUACAAGAGAUUGAUAGACUCGCUUGAUUUGGGCUUGAGUUAUGUGUCAAAAUCAAGUUAAAACAAUACAUAGCCUAUGGUUGUAGACAAAUGUGUUGGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO110变异链球菌(Streptococcus mutans)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGACCTATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCAGTAGCTGCCTAAAAACCAGCCTGTGTGATCAATAACAAATTGCTTGTGTTTGTTGATTGGTCTTATTGTTAACAAGCTACGTTAGAACTGAGTCAGGCTGTTCTAAAAGAGTTCTACTGACTCGCATCGTTAGAGTTTGAGTTATGTATTGTAACGGTGTTAAATAAACACATAACCTATAGTTGTAGACAAATGGGTTAGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO111变异链球菌(Streptococcus mutans)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGACCUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAGUAGCUGCCUAAAAACCAGCCUGUGUGAUCAAUAACAAAUUGCUUGUGUUUGUUGAUUGGUCUUAUUGUUAACAAGCUACGUUAGAACUGAGUCAGGCUGUUCUAAAAGAGUUCUACUGACUCGCAUCGUUAGAGUUUGAGUUAUGUAUUGUAACGGUGUUAAAUAAACACAUAACCUAUAGUUGUAGACAAAUGGGUUAGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO112肺炎链球菌(Streptococcus pneumoniae)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCGTGTGGCGACGTAAACGCTCAGTTAAATATAACTGCAAAAAATAACACTTCTTACGCTCTAGCTGCCTAAAAACCAGCAGGCGTGACCCGATTTGGATTGCTCGTGTTCAATGACAGGTCTTATTATTAGCGAGATACGATTAAGCCTTGTCTAGCGGTTTGATAAGAGATTGATAGACTCGCAGTTTCTAGACTTGAGTTATGTGTCGAGGGGCTGTTAAAATAATACATAACCTATGGTTGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO113肺炎链球菌(Streptococcus pneumoniae)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCGUGUGGCGACGUAAACGCUCAGUUAAAUAUAACUGCAAAAAAUAACACUUCUUACGCUCUAGCUGCCUAAAAACCAGCAGGCGUGACCCGAUUUGGAUUGCUCGUGUUCAAUGACAGGUCUUAUUAUUAGCGAGAUACGAUUAAGCCUUGUCUAGCGGUUUGAUAAGAGAUUGAUAGACUCGCAGUUUCUAGACUUGAGUUAUGUGUCGAGGGGCUGUUAAAAUAAUACAUAACCUAUGGUUGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO114化脓链球菌(Streptococcus pyogenes)ssrAGGGGTTGTTACGGATTCGACAGGCATTATGAGGCATGTTTTGCGTCCCATCGGCAGATGTAAATTGCCAGTTAAATATAACTGCAAAAAATACAAACTCTTACGCTTTAGCTGCCTAAAAACCAGCTAGCGTGACTTCTACAAGATTGCTTGTGTCCTGTTAGAAGTCTCAAAATAGCAAGCTACGGTTACGAAATTGTCTAGTTTCGTGACAAGAGATTGATAGACTCGCAAACTAATGGCTTGAGTTATGTGTCTTTAGTTTGTTAAATGAAGACATAACCTATGGACGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCAGCTCCACCASEQ ID NO115化脓链球菌(Streptococcus pyogenes)tmRNAGGGGUUGUUACGGAUUCGACAGGCAUUAUGAGGCAUGUUUUGCGUCCCAUCGGCAGAUGUAAAUUGCCAGUUAAAUAUAACUGCAAAAAAUACAAACUCUUACGCUUUAGCUGCCUAAAAACCAGCUAGCGUGACUUCUACAAGAUUGCUUGUGUCCUGUUAGAAGUCUCAAAAUAGCAAGCUACGGUUACGAAAUUGUCUAGUUUCGUGACAAGAGAUUGAUAGACUCGCAAACUAAUGGCUUGAGUUAUGUGUCUUUAGUUUGUUAAAUGAAGACAUAACCUAUGGACGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCAGCUCCACCA SEQ ID NO116Synechococcus sp.PCC6301 ssrAGGGGCTGTAATGGTTTCGACGTGTTGGTGAATCCTTCACCGTGATTCAGGCCGAGAGGGAGTCCACTCTCGTAAATCCAGGCTCAACCAAAAGTAACTGCGAACAACATCGTTCCTTTCGCTCGTAAGGCTGCTCCTGTAGCTGCTTAAACGCCACAAACTTTCTGGCTCGAGCGTCTAGTCGTAGACTCCGTTAATACGCCTAGACTTAAACCCCCAACGGATGCTCGAGTGGCGGCCTCAGGTCCGTCCTCTCGCTAAGCAAAAACCTGAGCATCCCGCCAACGGGGATAATCGTTGGCTCCCGCACAGTGGGTCAACCGTGCTAAGCCTGTGAACGAGCGGAAAGTTACTAGTCAATGCGGACAGCGGTTCGATTCCGCTCAGCTCCACCASEQ ID NO117Synechococcus sp.PCC6301 tmRNAGGGGCUGUAAUGGUUUCGACGUGUUGGUGAAUCCUUCACCGUGAUUCAGGCCGAGAGGGAGUCCACUCUCGUAAAUCCAGGCUCAACCAAAAGUAACUGCGAACAACAUCGUUCCUUUCGCUCGUAAGGCUGCUCCUGUAGCUGCUUAAACGCCACAAACUUUCUGGCUCGAGCGUCUAGUCGUAGACUCCGUUAAUACGCCUAGACUUAAACCCCCAACGGAUGCUCGAGUGGCGGCCUCAGGUCCGUCCUCUCGCUAAGCAAAAACCUGAGCAUCCCGCCAACGGGGAUAAUCGUUGGCUCCCGCACAGUGGGUCAACCGUGCUAAGCCUGUGAACGAGCGGAAAGUUACUAGUCAAUGCGGACAGCGGUUCGAUUCCGCUCAGCUCCACCA SEQ ID NO118Synechocystis sp.PCC6803 ssrAGGGGCCGCAATGGTTTCGACAGGTTGGCGAAAGCTTGCCCGTGATACAGGTCGAGAGTGAGTCTCCTCTCGCAAATCAAAGGCTCAAAAAAAAGTAACTGCGAATAACATCGTCAGCTTCAAACGGGTAGCCATAGCAGCCTAGTCTGTAAAAGCTACATTTTCTTGTCAAAGACCGTTTACTTCTTTTCTGACTCCGTTAAGGATTAGAGGTTAACCCCAACGGATGCTTTGTTTGGCTCTTCTCTAGTTAGCTAAACAATCAAGACTCAGACTAGAGCATCCCACCATCAGGGATAATCGATGGTCCCCGTCCTAGGGCTAGAAGGACTAAACCTGTGAATGAGCGGAAAGTTAATACCCAGTTTGGACAGCAGTTCAATTCTGCTCGGCTCCACCASEQ ID NO119Synechocystis sp.PCC6803 tmRNAGGGGCCGCAAUGGUUUCGACAGGUUGGCGAAAGCUUGCCCGUGAUACAGGUCGAGAGUGAGUCUCCUCUCGCAAAUCAAAGGCUCAAAAAAAAGUAACUGCGAAUAACAUCGUCAGCUUCAAACGGGUAGCCAUAGCAGCCUAGUCUGUAAAAGCUACAUUUUCUUGUCAAAGACCGUUUACUUCUUUUCUGACUCCGUUAAGGAUUAGAGGUUAACCCCAACGGAUGCUUUGUUUGGCUCUUCUCUAGUUAGCUAAACAAUCAAGACUCAGACUAGAGCAUCCCACCAUCAGGGAUAAUCGAUGGUCCCCGUCCUAGGGCUAGAAGGACUAAACCUGUGAAUGAGCGGAAAGUUAAUACCCAGUUUGGACAGCAGUUCAAUUCUGCUCGGCUCCACCA SEQ ID NO120海栖热袍菌(Thermotoga maritima)ssrAGGGGGCGAACGGGTTCGACGGGGATGGAGTCCCCTGGGAAGCGAGCCGAGGTCCCCACCTCCTCGTAAAAAAGGTGGGACAAAGAATAAGTGCCAACGAACCTGTTGCTGTTGCCGCTTAATAGATAAGCGGCCGTCCTCTCCGAAGTTGGCTGGGCTTCGGAAGAGGGCGTGAGAGATCCAGCCTACCGATTCAGCTTCGCCTTCCGGCCTGAATCGGGAAAACTCAGGAAGGCTGTGGGAGAGGACACCCTGCCCGTGGGAGGTCCCTCCCGAGAGCGAAAACACGGGCTGCGCTCGGAGAAGCCCAGGGGCCTCCATCTTCGGACGGGGGTTCGAATCCCCCCGCCTCCACCA SEQ ID NO121海栖热袍菌(Thermotoga maritima)tmRNAGGGGGCGAACGGGUUCGACGGGGAUGGAGUCCCCUGGGAAGCGAGCCGAGGUCCCCACCUCCUCGUAAAAAAGGUGGGACAAAGAAUAAGUGCCAACGAACCUGUUGCUGUUGCCGCUUAAUAGAUAAGCGGCCGUCCUCUCCGAAGUUGGCUGGGCUUCGGAAGAGGGCGUGAGAGAUCCAGCCUACCGAUUCAGCUUCGCCUUCCGGCCUGAAUCGGGAAAACUCAGGAAGGCUGUGGGAGAGGACACCCUGCCCGUGGGAGGUCCCUCCCGAGAGCGAAAACACGGGCUGCGCUCGGAGAAGCCCAGGGGCCUCCAUCUUCGGACGGGGGUUCGAAUCCCCCCGCCUCCACCA SEQ ID NO122嗜热栖热菌(Thermus thermophilus)ssrAGGGGGTGAAACGGTCTCGACGGGGGTCGCCGAGGGCGTGGCTGCGCGCCGAGGTGCGGGTGGCCTCGTAAAAACCCGCAACGGCATAACTGCCAACACCAACTACGCTCTCGCGGCTTAATGACCGCGACCTCGCCCGGTAGCCCTGCCGGGGGCTCACCGGAAGCGGGGACACAAACCCGGCTAGCCCGGGGCCACGCCCTCTAACCCCGGGCGAAGCTTGAAGGGGGCTCGCTCCTGGCCGCCCGTCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACTACGCGCGTAGAGGCCCGCCGTAGAGACCTTCGGACGGGGGTTCGACTCCCCCCACCTCCACCASEQ ID NO123嗜热栖热菌(Thermus thermophilus)tmRNAGGGGGUGAAACGGUCUCGACGGGGGUCGCCGAGGGCGUGGCUGCGCGCCGAGGUGCGGGUGGCCUCGUAAAAACCCGCAACGGCAUAACUGCCAACACCAACUACGCUCUCGCGGCUUAAUGACCGCGACCUCGCCCGGUAGCCCUGCCGGGGGCUCACCGGAAGCGGGGACACAAACCCGGCUAGCCCGGGGCCACGCCCUCUAACCCCGGGCGAAGCUUGAAGGGGGCUCGCUCCUGGCCGCCCGUCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACUACGCGCGUAGAGGCCCGCCGUAGAGACCUUCGGACGGGGGUUCGACUCCCCCCACCUCCACCA SEQ ID NO124苍白密螺旋体(Treponema pallidum)ssrAGGGGATGACTAGGTTTCGACTAGGGATGTGGGGTGTTGCGCTGCAGGTGGAGTGTCGATCTCCTGATTCGGCGCCTTTATAACTGCCAATTCTGACAGTTTCGACTACGCGCTCGCCGCGTAATCGCGGGCCTGTGTTTGCGCTGCTCTGAGCGAACATATCGGCCCGACGCCAAACGGAGCTTGCTCTTACGTTGTGCACGGCGGACGTAGGGGGACTTTTGTCTGTGCTAAGACTCTGGCGCGTGCGGTGCAGGCCTAGCAGAGTCCGACAAACGCAGTACGCACCGCTAAACCTGTAGGCGCGCAGCACTCGCTCTTTAGGACGGGGGTTCGATTCCCCCCATCTCCACCASEQ ID NO125苍白密螺旋体(Treponema pallidum)tmRNAGGGGAUGACUAGGUUUCGACUAGGGAUGUGGGGUGUUGCGCUGCAGGUGGAGUGUCGAUCUCCUGAUUCGGCGCCUUUAUAACUGCCAAUUCUGACAGUUUCGACUACGCGCUCGCCGCGUAAUCGCGGGCCUGUGUUUGCGCUGCUCUGAGCGAACAUAUCGGCCCGACGCCAAACGGAGCUUGCUCUUACGUUGUGCACGGCGGACGUAGGGGGACUUUUGUCUGUGCUAAGACUCUGGCGCGUGCGGUGCAGGCCUAGCAGAGUCCGACAAACGCAGUACGCACCGCUAAACCUGUAGGCGCGCAGCACUCGCUCUUUAGGACGGGGGUUCGAUUCCCCCCAUCUCCACCA SEQ ID NO126霍乱弧菌(Vibrio cholerae)ssrAGGGGCTGATTCAGGATTCGACGGGAATTTTGCAGTCTGAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCTTAATACCCTGCTCAGAGCCCTTCCTCCCTAGCTTCCGCTTGTAAGACGGGGAAATCAGGAAGGTCAAACCAAATCAAGCTGGCGTGGATTCCCCCACCTGAGGGATGAAGCGCGAGATCTAATTCAGGTTAGCCATTCGTTAGCGTGTCGGTTCGCAGGCGGTGGTGAAATTAAAGATCGACTAAGCATGTAGTACCAAAGATGAATGGTTTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO127霍乱弧菌(Vibrio cholerae)tmRNAGGGGCUGAUUCAGGAUUCGACGGGAAUUUUGCAGUCUGAGGUGCAUGCCGAGGUGCGGUAGGCCUCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUACCCUGCUCAGAGCCCUUCCUCCCUAGCUUCCGCUUGUAAGACGGGGAAAUCAGGAAGGUCAAACCAAAUCAAGCUGGCGUGGAUUCCCCCACCUGAGGGAUGAAGCGCGAGAUCUAAUUCAGGUUAGCCAUUCGUUAGCGUGUCGGUUCGCAGGCGGUGGUGAAAUUAAAGAUCGACUAAGCAUGUAGUACCAAAGAUGAAUGGUUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO128鼠疫耶尔森氏菌(Yersinia pestis)ssrAGGGGCTGATTCTGGATTCGACGGGATTCGCGAAACCCAAGGTGCATGCCGAGGTGCGGTGGCCTCGTAAAAAACCGCAAAAAAAATAGTTGCAAACGACGAAAACTACGCACTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTGCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCTAAAAGAGCTCGTGTGGAAACCTTGCCTGGGGTGGAAGCATTAAAACTAATCAGGATAGTTTGTCAGTAGCGTGTCCATCCGCAGCTGGCCGGCGAATGTAATGATTGGACTAAGCATGTAGTGCCGACGGTGTAGTAATTTCGGACGGGGGTTCAAATCCCCCCAGCTCCACCA SEQ ID NO129鼠疫耶尔森氏菌(Yersinia pestis)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGUGCGGUGGCCUCGUAAAAAACCGCAAAAAAAAUAGUUGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUGCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCUAAAAGAGCUCGUGUGGAAACCUUGCCUGGGGUGGAAGCAUUAAAACUAAUCAGGAUAGUUUGUCAGUAGCGUGUCCAUCCGCAGCUGGCCGGCGAAUGUAAUGAUUGGACUAAGCAUGUAGUGCCGACGGUGUAGUAAUUUCGGACGGGGGUUCAAAUCCCCCCAGCUCCACCA SEQ ID NO130胎儿弯曲杆菌(Campylobacter fetus)ssrA，内部部分AGGAGTAA